NS-1 (P3/NS/1-Ag4.1) Cells
General information
Description | Resistant to 8-azaguanine, no proliferation in HAT-selection medium. |
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Organism | Mouse |
Disease | Myeloma |
Synonyms | P3/NSI/1-AG4-1, P3/NS1/1-Ag4-1, P3/NS1/1-AG4-1, P3/NS1/Ag4-1, P3 NS1 Ag4/1, P3 NS1 Ag4, P3.NS-1/1.Ag4.1, P3-NS/1-Ag4-1, P3-NS1/1-Ag4-1, P3-NS1/1Ag 4.1, P3/NS-1, NS1/1-Ag4.1, NS1-1 Ag4.1, NS-1-Ag4-1, NS1-Ag4/1, NS1-Ag 4/1, NS1-Ag4, P3x63NS1, NS-I/1, NSI/1-Ag4-1, NS-1, NS1, GM03573, GM-3573, GM03573A |
Characteristics
Gender | Female |
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Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | NS-1 (Cytion catalog number 400108) |
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Biosafety level | 1 |
Expression / Mutation
Products | The cells synthesise kappa-light chain of Immunoglobulin G (IgG1) but do not secrete |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 3 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
M_18-3: 19
M_4-2: 21.3,22.3
M_6-7: 12
M_3-2: 13,14
M_19-2: 13
M_7-1: 26.2,28.2
M_1-1: 16,17
M_8-1: 13
M_2-1: 15,16,17
M_15-3: 22,3
M_6-4: 17,18,19
M_11-2: 17,18
M_1-2: 16,17
M_17-2: 16,17
M_12-1: 15,16
M_5-5: 13,14
M_X-1: 25,26
M_13-1: 16.2,17.2
Human D4/D8: -
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