NRK-EGFP-H2B Cells
$540.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | This clonal stable cell line was generated by transfection of a circular plasmid followed by drug resistance selection. Add G418 to culture medium at a final concentration of 0.5 mg/ml. |
---|---|
Organism | Rat |
Tissue | Kidney |
Synonyms | NRK EGFP-H2B |
Characteristics
Morphology | Fibroblast-like cells with fusiform shape |
---|---|
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | NRK-EGFP-H2B (Cytion catalog number 500724) |
---|---|
Biosafety level | 1 |
Depositor | Dr. J. Ellenberg, EMBL Heidelberg |
Expression / Mutation
Receptors expressed | Epidermal growth factor (EGF), multiplication stimulating activity (MSA) |
---|---|
Protein expression | EGFP-H2B: Location/Gene: 1..589 / Pcmv, 613..1329 / EGFP, 1387..1764 / H2B, 3001..3795 / KanR/NeoR |
Products | Epidermal growth factor (EGF), multiplication stimulating activity (MSA), CMV Promotor Histone H2B, Neomycin, Phosphotransferase |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS, 0.5 mg/ml G418 |
Passaging solution | Accutase |
Subculturing | Discard the old medium and wash the cells with PBS. Add a freshly prepared 0.025% trypsin/0.02% EDTA solution heated to 37 degrees Celsius and wait until the cells detach, which usually takes about 5 minutes. Neutralize the trypsin by adding fresh medium, then transfer the cell mixture to a tube and centrifuge. After centrifugation, remove the supernatant, resuspend the cell pellet in fresh culture medium, and transfer the suspension to new flasks. Incorporate G418 into the culture medium to achieve a final concentration of 0.5 mg/ml |
Split ratio | A ratio of 1:3 to 1:4 is recommended |
Seeding density | 2 to 4 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|