NRK-52E Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305196

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The NRK-52E cell line, derived from the normal kidney of a rat, is an epithelioid cell line representing proximal tubular epithelial cells. This cell line is widely used in nephrology research, especially for studies on renal physiology, toxicology, and pathophysiology. NRK-52E cells display characteristic epithelial morphology with tight junctions, making them suitable for in vitro modeling of renal tubular function and barrier integrity. NRK-52E cells have been instrumental in studying mechanisms of apoptosis, cellular repair, and ion transport. For instance, the cell line has been used to investigate the effects of okadaic acid, a protein phosphatase inhibitor, revealing its role in inducing apoptotic pathways involving chromatin condensation, calcium influx, and mitochondrial changes. These studies have provided insights into the regulation of renal cell death and survival mechanisms during injury or disease. Furthermore, NRK-52E cells have been used to assess renal epithelial ion transport and barrier properties under various experimental setups, such as microfluidic systems that mimic physiological flow conditions. This includes research on sodium chloride reabsorption and transepithelial electrical resistance, which are critical for understanding electrolyte and water balance in renal physiology. These characteristics make NRK-52E a robust model for exploring renal tubular cell biology and therapeutic interventions in kidney diseases.
Organism	Rat
Tissue	Kidney
Synonyms	NRK 52E, NRK52E, NRK clone 52E, Normal Rat Kidney-52E, NRK-E52

Breed/Subspecies	Osborne-Mendel
Morphology	Epithelial
Growth properties	Adherent

Citation	NRK-52E (Cytion catalog number 305196)
Biosafety level	1
NCBI_TaxID	10116
CellosaurusAccession	CVCL_0468

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305196-200625	Certificate of Analysis	18. Aug. 2025	305196

NRK-52E Cells

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Certificate of Analysis (CoA)