NFS-60 Cells
General information
Description | NFS-60 is a murine myeloblastic cell line established from leukemic cells obtained after infection of (NFS x DBA/2) F1 adult mice with Cas Br-M murine leukemia virus. NFS-60 cells are dependent on IL3 for growth and maintenance of viability in vitro. These cells are used to assay murine and human G-CSF. This bipotential murine hematopoietic cell line is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin. |
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Organism | Mouse |
Tissue | Blood |
Disease | Leukemia |
Synonyms | M-NFS-60, NFS 60, NFS60 |
Characteristics
Cell type | Lymphoblast |
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Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | NFS-60 (Cytion catalog number 400301) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS, 33 IU/ml IL-3 |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | Start cultures at 5 x 10^4 viable cells/ml. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
M_18-3: 16
M_4-2: 19.3,20.3
M_6-7: 11,12
M_3-2: 13,14
M_19-2: 11,12
M_7-1: 28,29
M_1-1: 10,16
M_8-1: 15,16
M_2-1: 9,16
M_15-3: 20.3,21.3
M_6-4: 15.3,18
M_11-2: 17,18
M_1-2: 17
M_17-2: 13,15
M_12-1: 16,20
M_5-5: 14,15
M_X-1: 25,27
M_13-1: 13,14.2
Human D4/D8: -
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