NCI-H82 Cells
General information
Description | The NCI-H82 cell line was derived by A.F. Gazdar and associates in 1978 from the pleural fluid of a patient with small cell cancer of the lung. The morphology of the original tumor was not characteristic of SCLC. The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase. It does not have detectable levels of L-DOPA decarboxylase or bombesin. The cells produce an abnormally sized p53 mRNA (3.7 kb). C-myc DNA sequences are amplified about 25 fold, and there is a 24 fold increase in c-myc RNA relative to normal cells. The cells are reported to express functional ANP receptors, but treatment with ANP does not alter their growth pattern. The cells stain positively for neurofilaments and vimentin. There is expression of v-fes, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs. |
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Organism | Human |
Tissue | Lung |
Disease | Lung small cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | NCI-H-82, H82, H-82, NCI H82, NCIH82, H82sclc |
Characteristics
Age | 41 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Aggregates in suspension. The cells grow in very large aggregates, which are the only viable cell population in the culture. |
Identifiers / Biosafety / Citation
Citation | NCI-H82 (Cytion catalog number 300442) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Insulin-like growth factor II receptor (IGF II), atrial natriuretic peptide (ANP) |
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Protein expression | p53 positive |
Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 1, Me-2, 1, AK-1, 1, GLO-1, 1, Phenotype Frequency Product = 0.0082 |
Tumorigenic | Yes, forms transplantable tumors with non-typical SCLC histology in nude mice |
Karyotype | This is a near triploid human cell line. The modal chromosome number is 58, occurring at 44% with polyploidy at 3%. Each cell had two copies of a normal x chromosome. The Y chromosome was not detected in Q banded preparations. |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | A ratio of 1:2 to 1:5 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 11
D13S317: 8
D16S539: 12
D5S818: 12
D7S820: 10,13
TH01: 9,9.3
TPOX: 11
vWA: 14
D3S1358: 17
D21S11: 28,30
D18S51: 14,18
Penta E: 11,12
Penta D: 10,12
D8S1179: 13
FGA: 24,25
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