NCI-H69 Cells
General information
Description | This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate. The line can be adapted to grow in shaker flask or spinner flask systems. These cells are not resistant to Adriamycin. |
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Organism | Human |
Tissue | Lung |
Disease | Lung small cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | NCI-H-69, NCI H69, H69, H-69, NCIH69, NCI-HUT-69, H69/P, NCI-H69C, H69C, H69c |
Characteristics
Age | 55 years |
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Gender | Male |
Ethnicity | Caucasian |
Growth properties | Floating aggregates |
Identifiers / Biosafety / Citation
Citation | NCI-H69 (H69) (Cytion catalog number 300185) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Insulin-like growth factor II receptor (IGF II) |
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Protein expression | p53 negative, cytokeratins positive |
Isoenzymes | G6PD, B, PGM1, 2, PGM3, 1, ES-D, 2, Me-2, 1, AK-1, 1, GLO-1, 1-2, Phenotype Frequency Product: 0.00006 |
Tumorigenic | Forms tumors with typical small cell carcinoma histology |
Karyotype | Aneuploid, with 3p deletion. Range = 40 to 73 |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 69 hours |
Subculturing | Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 x 10^5 cells/mL |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
TH01: 8,9
TPOX: 10
vWA: 16,17
D3S1358: 16
D21S11: 30,31.2
D18S51: 12
Penta E: 12
Penta D: 9,11
D8S1179: 13
FGA: 24
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HLA alleles |
A*: 02:01:01, 23:01:01
B*: 01:01:01, 02.01.1900 03:01
C*: 07:01:01, 14:02:01
DRB1*: 04:04:01, 04:05:01
DQA1*: 03:01:01, 03:03:01
DQB1*: 03:02:01
DPB1*: 01:01:01G, 03:01:01G
E: 01:01:01
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