NCI-H2009 Cells

USD$540.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305283

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The NCI-H2009 cell line is derived from a human non-small cell lung carcinoma (NSCLC), specifically an adenocarcinoma. This cell line is extensively used in lung cancer research to study the molecular and cellular mechanisms underlying adenocarcinoma, the most common subtype of NSCLC. NCI-H2009 cells are valuable for investigating genetic mutations, signal transduction pathways, and therapeutic responses associated with lung adenocarcinoma. NCI-H2009 cells exhibit an epithelial morphology and express markers characteristic of lung adenocarcinoma, including cytokeratins and carcinoembryonic antigen (CEA). They harbor genetic alterations frequently observed in NSCLC, such as mutations in the KRAS gene, which is pivotal in cell signaling, growth, and survival. Researchers utilize NCI-H2009 cells to explore key signaling pathways involved in lung cancer progression, such as the EGFR, KRAS, and PI3K/Akt pathways. These cells are also employed in high-throughput drug screening assays and preclinical testing of chemotherapeutic agents, targeted therapies, and immunotherapies. Additionally, NCI-H2009 cells are used to study mechanisms of drug resistance and to develop strategies to overcome it. The relevance of the NCI-H2009 cell line in lung adenocarcinoma research highlights its importance in advancing our understanding of lung cancer biology and in developing new and more effective treatment approaches for patients with NSCLC.
Organism	Human
Tissue	Lung
Disease	Adenocarcinoma
Metastatic site	Lymph node
Synonyms	H2009, H-2009, NCIH2009

Age	68 years
Gender	Female
Ethnicity	European
Morphology	Epithelial
Growth properties	Adherent

Citation	NCI-H2009 (Cytion catalog number 305283)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1514

Viruses	Transformant: Epstein-Barr virus (EBV)
Mutational profile	Mutation: B2M, p.Met1Val (c.1A>G), heterozygous; Mutation: B2M, p.Gln28Ter (c.82C>T), heterozygous; Mutation: KRAS, p.Gly12Ala (c.35G>C), heterozygous; Mutation: TERT, c.1-124C>T (c.228C>T) (C228T); Mutation: TP53, p.Arg273Leu (c.818G>T), homozygous

Culture Medium	HITES medium supplemented The base medium for this cell line is DF12. To make the complete growth medium, add the following components to the base medium: 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30 nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) Extra 2 mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.