NCI-H1373 Cells

USD$540.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Product number: 305597

Safety data sheet

Product sheet

Description	The NCI-H1373 cell line is a human non-small cell lung carcinoma (NSCLC) model harboring the KRAS G12C mutation, which is frequently associated with oncogenesis in lung adenocarcinoma. This mutation results in constitutive activation of downstream signaling pathways such as the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways, driving tumor proliferation and survival. NCI-H1373 is extensively used in research to evaluate the efficacy of novel therapeutic agents targeting KRAS G12C and related oncogenic pathways. In preclinical studies, NCI-H1373 has been employed to assess the activity of emerging KRAS G12C inhibitors such as ASP2453. This inhibitor demonstrated potent, selective, and covalent binding to the mutant KRAS protein, suppressing KRAS-mediated signaling and tumor cell proliferation in vitro. The cell line was also used in xenograft models, where ASP2453 induced significant tumor regression. This activity underscores NCI-H1373's utility in understanding the therapeutic potential and mechanisms of novel KRAS inhibitors. Additionally, NCI-H1373 has been utilized in genome-wide CRISPR knockout screens to explore mechanisms of resistance to PARP7 inhibitors. The results revealed that loss of aryl hydrocarbon receptor (AHR) signaling is associated with resistance to PARP7 inhibition, suggesting potential combination strategies. Such studies demonstrate the cell line's role in identifying resistance pathways and optimizing targeted therapies for KRAS G12C-mutated NSCLC.
Organism	Human
Tissue	Lung
Disease	Adenocarcinoma
Synonyms	H1373, H-1373, NCIH1373

Age	56 years
Gender	Male
Ethnicity	African American
Morphology	Epithelial-like
Growth properties	Adherent

Citation	NCI-H1373 (Cytion catalog number 305597)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1465

Mutational profile	Mutation: KRAS, p.Gly12Cys (c.34G>T), homozygous; Mutation: TP53, p.Pro47Leu (c.140C>T), homozygous

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with TrypLE Express, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

NCI-H1373 Cells

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Organism	Human
Tissue	Stomach
Disease	Gastric tubular adenocarcinoma