MX-1 Cells
General information
Description | The Mx-1 cell line has been established as in vitro culture from the Mx-1 tumor xenograft model of breast carcinoma tissue. |
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Organism | Human |
Tissue | Breast |
Disease | Adenocarcinoma, Infiltrating duct carcinoma (IDC) |
Synonyms | Mx1, MxI |
Characteristics
Age | 29 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Mx-1 (Cytion catalog number 300296) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Estrogen (oestrogen) receptor (-) |
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Protein expression | p53 (-) |
Tumorigenic | Yes, in nude mice |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 30 to 35 hours |
Subculturing | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 degree Celsius for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. Do not allow the cells to become confluent, subculture once per week. Note: The cells do not form a confluent monolayer. Subculture when a dense layer of cells is observed macroscopically. |
Split ratio | A ratio of 1:2 to 1:3 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Fast |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11
D13S317: 11,12
D16S539: 12
D5S818: 12
D7S820: 11,12
TH01: 7,9
TPOX: 7,8
vWA: 17,18
D3S1358: 15
D21S11: 29,30,31,32
D18S51: 12,16
D8S1179: 11,12,13
FGA: 20
D2S1338: 19
D19S433: 13,15.2,16.2
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HLA alleles |
A*: 11:01:01
B*: 35:01:01
C*: 04:01:01
DRB1*: 01:03:01
DQA1*: 01:01:01
DQB1*: 05:01:01
DPB1*: 04:01:01
E: 01:01:01
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