MOLT-3 Cells
General information
Description | The T-cell lines MOLT-3 and MOLT-4 are derived from the leukemic cells of a patient with acute lymphoblastic leukaemia whilst in relapse. The cells should be handled under laboratory containment level 2 conditions. |
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Organism | Human |
Tissue | Peripheral blood |
Disease | Acute lymphoblastic leukemia (ALL) |
Synonyms | Molt-3, MOLT 3, Molt 3, MOLT3, Molt3 |
Characteristics
Age | 19 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | T lymphocyte |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | MOLT-3 (Cytion catalog number 300116) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD1(+), CD5(+), CD7(+), CD11a(+) (Greenberg et al. 1988). |
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Karyotype | hypertetraploid |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS, 1 mM sodium pyruvate, 1% NEAA |
Doubling time | 24 to 48 hours |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 0.5 to 1 x 10^5 cells/ml |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11,12,13
D13S317: 12,13
D16S539: 11,14,15
D5S818: 12,13
D7S820: 7,8,9,10
TH01: 6,8
TPOX: 8
vWA: 17,18
D3S1358: 15,16,17
D21S11: 29,30,31,32
D18S51: 12,13,16,17
Penta E: 14,16
Penta D: 8,13
D8S1179: 9,13,14,15
FGA: 19,21,25
D1S1656: 15.3,16,16.3
D6S1043: 14,15,16
D2S1338: 23,24
D12S391: 17,19,20
D19S433: 14,15,16
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HLA alleles |
A*: 01:01:01, 25:01:01
B*: 18:01:01, 57:01:01
C*: 06:02:01, 12:03:01
DRB1*: 07:01:01, 12:01:01
DQA1*: 02:01:01, 05:05:01
DQB1*: 02:02:01, 03:01:01
DPB1*: 02:01:02
E: 01:01:01, 01:xx
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