MML-1 Cells
General information
Description | This cell line was established as in vitro culture from a solid tumor which was induced in nu/nu nude mice using deep frozen tumor tissue. |
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Organism | Human |
Tissue | Skin |
Disease | Melanoma |
Synonyms | MML1 |
Characteristics
Age | Unspecified |
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Gender | Unspecified |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | MML-1 (Cytion catalog number 300288) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 positive |
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Tumorigenic | Yes, in nude mice |
Reverse transcriptase | negative |
Mutational profile | V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot). |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:5 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10
D13S317: 8,13
D16S539: 10,11
D5S818: 10,12
D7S820: 10,12
TH01: 6,10
TPOX: 11
vWA: 17,18
D3S1358: 17
D21S11: 31
D18S51: 13,14
Penta E: 7,11
Penta D: 14
D8S1179: 13,14
FGA: 23
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