MHH-ES1 Cells
General information
Description | Established from the malignant ascites (with peritoneal metastasis). |
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Organism | Human |
Tissue | Bone |
Disease | Ewing's Sarcoma |
Metastatic site | Ascites |
Synonyms | MHH-ES-1, MHHES1 |
Characteristics
Age | 12 years |
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Gender | Male |
Ethnicity | Turkish |
Morphology | Small round cells |
Growth properties | Adherent, clusters |
Identifiers / Biosafety / Citation
Citation | MHH-ES1 (Cytion catalog number 300136) |
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Biosafety level | 1 |
Depositor | Hartmann |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Seeding density | 1 to 2 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 11
D13S317: 8
D16S539: 11
D5S818: 13
D7S820: 9,11
TH01: 8,9
TPOX: 8
vWA: 16,17
D3S1358: 15,16
D21S11: 28,32.2
D18S51: 14,16
Penta E: 11,15
Penta D: 11,12
D8S1179: 11,13
FGA: 22
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HLA alleles |
A*: 01:01:01, 68:01:01
B*: 40:01:02, 49:01:01
C*: 01:02:01, 07:01:01
DRB1*: 07:01:01, 11:01:01
DQA1*: 02:01:01, 05:05:01
DQB1*: 03:01:01, 03:03:02G
DPB1*: 10:01:01, 13:01:01
E: 01:01:01, 01:03:01
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