MH-TC-5123 Cells
General information
Description | In vitro established from the Buffalo-rat hepatoma by CLS Cell lines Service. |
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Organism | Rat |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Characteristics
Age | 10 months |
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Gender | Unspecified |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | MH-TC-5123 (Cytion catalog number 500287) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in Buffalo-rat |
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Viruses | RAP-test negative. |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
Rat_D1Wox31: 104
Rat_D2Wox37: 156
Rat_D19Wox11: 220
Rat_D10Wox8: 266
Rat_D4Wox7: 149,153
Rat_D2Wox27: 207
Rat_D5Rat33: 126,128
Rat_D10Wox11: 165
Rat_D1Wox23: 222
Rat_D12Wox1: 402
Rat_D6Wox2: 104
Rat_D8Wox7: 185
Rat_D6Cebr1: 233,235
SRY: x,x
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