MH-3924A Cells
General information
Description | In vitro established from the ACI-rat hepatoma (Cell Lines Service). |
---|---|
Organism | Rat |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Synonyms | MH 3924A, MH3924A, MH-3924 A, MH 3924 A, 3924A, Morris hepatoma 3924A, MH-3924, MH3924, MH 3924 |
Characteristics
Age | 16 months |
---|---|
Gender | Unspecified |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | MH-3924A (Cytion catalog number 500286) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in ACI-rat |
---|---|
Viruses | RAP-test negative by PCR for: Adenovirus FL, Adenovirus K87, Hantavirus, Kilham rat virus, Lmyfocytair choriomeningitis virus, Mycoplasma pulmonis, Pneumonia virus of mice, Rat corona virus / Sialoacryoadenitis virus, Rat parvo virus, Reovirus type 3, Sendai virus, Theiler-s encephalomyelitis virus, Toolan-s H-1 virus. |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 25 to 35 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | Start culture using the complete contents of the cryovial in 2xT25 cell culture flasks. The cells will recover within 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
Rat_D1Wox31: 100
Rat_D2Wox37: 156
Rat_D19Wox11: 228
Rat_D10Wox8: 266,270
Rat_D4Wox7: 141,145
Rat_D2Wox27: 223
Rat_D5Rat33: 120,122
Rat_D10Wox11: 156,159
Rat_D1Wox23: 226,234
Rat_D12Wox1: 410
Rat_D6Wox2: 100,112,120
Rat_D8Wox7: 161,182
Rat_D6Cebr1: 239
SRY: x,x
|