ME-180 Cells
General information
Description | The ME-180 cell line, characterized by its epithelial morphology, is derived from the uterine cervix of a 66-year-old Caucasian female with epidermoid carcinoma. ME-180 cells present a complex karyotype, ranging from hyperdiploid to hypohexaploid, featuring notable chromosomal abnormalities such as dicentrics, fragmentation, and various markers, reflecting its genetic instability. ME180 cells are tumorigenic, capable of forming well-differentiated epidermoid carcinoma (grade I) in nude mice, indicative of its origin from a highly invasive squamous cell carcinoma lacking significant keratinization. ME-180 cells are particularly valuable in cancer and infectious disease research, including studies on sexually transmitted diseases. A distinctive aspect of ME-180 is its response to tumor necrosis factor alpha (TNF alpha), which inhibits cell growth, offering insights into the cell line's interaction with cytokines and potential therapeutic targets. Additionally, these cells harbor human papillomavirus (HPV) DNA, showing closer homology to HPV-68 than to HPV-18, which adds a layer of relevance to studies on viral oncogenesis and the development of antiviral strategies. |
---|---|
Organism | Human |
Tissue | Uterus, Cervix |
Disease | Epidermoid Carcinoma |
Metastatic site | Omentum |
Synonyms | Me-180, ME 180, ME180 |
Characteristics
Age | 66 years |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Cell type | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | ME-180 (Cytion catalog number 300196) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Viruses | HPV positive |
---|
Handling
Culture Medium | McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 11,11
D13S317: 11,13
D16S539: 12,13
D5S818: 12,12
D7S820: 9,10
TH01: 8,9.3
TPOX: 8,10
vWA: 15,17
D3S1358: 16,16
D21S11: 30,31
D18S51: 12,12
Penta E: 12,14
Penta D: 9,14
D8S1179: 14,14
FGA: 23,23
|