MC3T3-E1 Subclone 24 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305186

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	MC3T3-E1 Subclone 24 cells expressly represent a preosteoblast cell type, which plays a crucial role in bone formation. Morphologically, they exhibit a fibroblast-like appearance, characterized by their elongated shape and spindle-like structures. This particular subclone is derived from the calvaria tissue, a skull region contributing to bone formation. One of the critical applications of MC3T3-E1 Subclone 24 Cells lies in 3D cell culture, where researchers can study the behavior and interactions of these cells within a three-dimensional environment. This method offers a more physiologically relevant model than traditional two-dimensional cell cultures, allowing for a better understanding of the intricate processes involved in bone formation. While these cells possess numerous advantages, it's important to note their specific characteristics. MC3T3-E1 Subclone 24 Cells have been observed to exhibit poor osteoblast differentiation when exposed to ascorbic acid, a key component for promoting bone cell growth. Furthermore, they do not form a mineralized extracellular matrix, a crucial step in creating bone tissue. The doubling time of MC3T3-E1 Subclone 24 Cells is approximately 90.5 hours.
Organism	Mouse
Tissue	Bone
Applications	3D cell culture, Differentiation studies

Breed/Subspecies	C57BL/6
Age	1 day
Gender	Unspecified
Morphology	Fibroblast
Cell type	Osteoblast
Growth properties	Adherent

Citation	MC3T3-E1 Subclone 24 (Cytion catalog number 305186)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_5438

Receptors expressed	Parathyroid hormone-related protein (PTHrP) receptor
Protein expression	Collagen, bone sialoprotein (BSP), osteocalcin (OCN), parathyroid hormone (PTH)
Tumorigenic	Yes, in immunosuppressed mice

Culture Medium	Alpha MEM, w: 2.0 mM stable Glutamine, w: Ribonucleosides, w: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3, w/o: Ascorbic acid (GIBCO, Catalog No. A1049001. We do not supply this product; please consider other suppliers. Please let us know if you need further assistance.)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305186-120224	Certificate of Analysis	23. May. 2025	305186

MC3T3-E1 Subclone 24 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)