MARC-145 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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USD$800.00*

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cryovial

Product number: 305006

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The MARC-145 cell line is a monkey kidney epithelial cell line derived from the African green monkey (Cercopithecus aethiops). This cell line is particularly notable for its use in virology, especially in the propagation of porcine reproductive and respiratory syndrome virus (PRRSV), a significant pathogen in the swine industry. MARC-145 cells have been instrumental in studying PRRSV due to their high susceptibility to the virus, making them a valuable tool for viral isolation, propagation, and vaccine development. MARC-145 cells are characterized by their epithelial morphology and robust growth in vitro, which facilitates large-scale production of PRRSV for research purposes. Additionally, they have been used to explore the mechanisms of PRRSV infection, including virus entry, replication, and host-pathogen interactions. This cell line has also been utilized in the development and testing of antiviral compounds and PRRSV vaccines, contributing significantly to efforts aimed at controlling this economically important disease in pigs.
Organism	Chlorocebus pygerythrus (Vervet monkey)
Tissue	Embryonic kidney
Synonyms	Marc-145, MARC 145, Marc 145, MARC145, Marc145, Meat Animal Research Center-145

Age	Foetus
Morphology	Epithelial
Growth properties	Adherent

Citation	MARC-145 (Cytion catalog number 305006)
Biosafety level	1
NCBI_TaxID	60710
CellosaurusAccession	CVCL_4540

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305006-140723	Certificate of Analysis	22. Jan. 2026	305006
305006-070425	Certificate of Analysis	23. Jan. 2026	305006

MARC-145 Cells

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Certificate of Analysis (CoA)