MA-104 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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USD$550.00*

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cryovial

Product number: 305007

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The MA-104 cell line is derived from rhesus monkey kidney epithelial cells and is widely used in virology and vaccine production research. These cells exhibit a typical epithelial morphology, adhering tightly to the substrate and forming a monolayer. Due to their origin, MA-104 cells are particularly permissive to the replication of various viruses, including rotaviruses, polioviruses, and reoviruses, making them an essential tool in virological studies, especially in the propagation and isolation of these pathogens. Their high susceptibility to viral infection allows for efficient viral growth, which is crucial for vaccine development and testing. In addition to their role in virology, MA-104 cells are also employed in studies focusing on cell biology and physiology, particularly those investigating kidney function and epithelial cell behavior. These cells have been instrumental in understanding the mechanisms of viral entry, replication, and the host-cell response to infection. Researchers also utilize MA-104 cells to study protein expression and post-translational modifications due to their ability to support high levels of protein production.
Organism	Chlorocebus pygerythrus (Vervet monkey)
Tissue	Kidney
Synonyms	Ma-104, MA 104, MA104, Microbiological Associates-104

Age	Fetus
Morphology	Epithelial
Growth properties	Adherent

Citation	MA-104 (Cytion catalog number 305007)
Biosafety level	1
NCBI_TaxID	60710
CellosaurusAccession	CVCL_3845

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305007-141222	Certificate of Analysis	22. Jan. 2026	305007

MA-104 Cells

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Certificate of Analysis (CoA)