M-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305261

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The M-1 cell line is a well-characterized epithelial model derived from the kidney of a transgenic adult mouse. Specifically, M-1 cells originate from the cortical collecting duct epithelium and retain many differentiated features of this nephron segment. These cells express markers typical of cortical collecting duct cells, including epithelial sodium channels (ENaC), aquaporins, and tight junction proteins, making them a widely used in vitro model for renal physiology, ion transport, and epithelial polarity studies. Functionally, M-1 cells exhibit high transepithelial resistance and vectorial ion transport properties, which are critical for studying aldosterone-regulated sodium reabsorption and vasopressin-mediated water transport. According to the foundational characterization by Stoos et al., M-1 cells form polarized monolayers on permeable supports and show appropriate responsiveness to hormonal stimuli, such as dexamethasone and aldosterone, that regulate transport protein expression and activity. These features make M-1 cells particularly valuable in dissecting mechanisms of electrolyte handling and cellular signaling in kidney epithelial cells. Moreover, M-1 cells have been validated in more recent studies, including genetic authentication using STR profiling for mouse cell lines. This underscores their continued relevance and reliability in contemporary renal physiology research. Their ability to recapitulate in vivo-like behaviors under controlled conditions has established them as a standard in studies on epithelial function, nephrotoxicity, and renal disease modeling.
Organism	Mouse
Tissue	Kidney, cortical collecting duct
Synonyms	M1-CCD

Breed/Subspecies	Tg(SV40E)Bri/7 transgenic
Age	Unspecified
Gender	Unspecified
Morphology	Epithelial
Growth properties	Adherent

Citation	M-1 (Cytion catalog number 305261)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_8786
GMO Status	GMO-S1: This murine collecting duct cell line (M-1) contains the early region of SV40 from a transgenic mouse line (Tg(SV40E)Bri7), supporting stable immortalization. The construct is endogenously integrated in the transgenic background. This classification applies only within Germany and may differ elsewhere.

Viruses	Simian virus 40 (SV40)

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Supplements	Supplement the medium with 5% FBS, 5 µM dexamethasone
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305261-060924	Certificate of Analysis	23. May. 2025	305261

M-1 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)