LXF-289 Cells
General information
Description | In vitro etablished from the primary lung adenocarcinoma of a 62 year-old male. |
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Organism | Human |
Tissue | Lung |
Disease | Adenocarcinoma |
Synonyms | LxF289, LxF 289, LxF 289L |
Characteristics
Age | 62 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | LxF-289 (Cytion catalog number 300269) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice |
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Reverse transcriptase | negative |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/ml |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | 24 to 48 hours |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 12,13
D13S317: 9,11
D16S539: 13
D5S818: 9,10
D7S820: 10,11
TH01: 6,9.3
TPOX: 11
vWA: 17,18
D3S1358: 15,18
D21S11: 30,31
D18S51: 14
Penta E: 10,20
Penta D: 10,13
D8S1179: 13
FGA: 24,25
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