LS174T Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300392

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The LS147T cell line is a variant of LS-180, both of which are derived from a Duke's type B adenocarcinoma of the colon in a 58-year-old White female patient. The original LS-180 line was established by culturing the minced tumor tissue for 10 months. LS-147T, along with its parent line, is notable for its expression of multiple oncogenes including myc, myb, ras, and fos, while being negative for others like sis, abl, and ros. This line also expresses high levels of carcinoembryonic antigen (CEA), interleukin 6 (IL-6), and interleukin 10 (IL-10), which are important markers and potential targets in colorectal cancer research. These cells exhibit several key characteristics of colonic epithelial cells, including abundant microvilli and intracytoplasmic mucin vacuoles, which are features typically associated with secretory cells in the colonic mucosa. Electron microscopy studies have confirmed these structural details, further supporting their origin and differentiation status. Importantly, LS-147T cells have been shown to be tumorigenic in immunodeprived mice, consistently producing tumors when inoculated subcutaneously at high cell densities, thus affirming their malignant potential. Moreover, the LS-147T cell line is particularly valuable in studies focusing on the molecular and immunological aspects of colorectal cancer. It has been reported that this line is easier to subculture compared to its parent line, LS-180, making it a more practical choice for long-term studies. The robust production of CEA by these cells, which is significantly higher than that of other established lines like HT-29, makes LS-147T a critical model for understanding tumor marker dynamics and exploring targeted therapies in colorectal cancer.
Organism	Human
Tissue	Colon
Disease	Adenocarcinoma
Synonyms	Ls174T, LS174t, Ls-174-T, LS-174-T, LS 174 T, LS174T, Ls-174T, LS 174T, LS-174, LS174

Age	58 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent

Citation	LS174T (Cytion catalog number 300392)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1384

Protein expression	Colon Antigen 3 +, CEA +, p53 -, GFAP -, mRNA expression +
Antigen expression	HLA A2, B13, B50, Blood type O
Isoenzymes	ADA, 1: G6PD, B, PGM1, 1, PGM3, 2, PGD, A, ES-D, 1, PEP-D, 1
Oncogenes	Myc +, myb + , ras +, fos +, p53 +, sis -, abl -, ros -, src -
Tumorigenic	Yes, in nude mice
Reverse transcriptase	Negative
Products	Carcinoembryonic antigen (CEA) 1944 ng/106 cells in 10 days, mucin, interleukin-10 (IL-10), interleukin-6 (IL-6)
Mutational profile	LS-174T cells carry a mutation in codon 12 of Kras gene: GGT(Wt Gly) >GAT(Asp)
Karyotype	45,x with one x chromosome missing but no other chromosomal aberrations

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	5 to 8 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300392-070225	Certificate of Analysis	23. May. 2025	300392
300392-410, -412, -414	Certificate of Analysis	05. Dec. 2025	300392
300392-512	Certificate of Analysis	23. May. 2025	300392

LS174T Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)