LMH Cells
General information
Description | LMH cells, derived from a Leghorn male hepatoma, are a versatile cell line widely used in biological research. Tomoyuki Kitagawa established them in 1981 at the Cancer Institute in Tokyo, Japan. These cells have an epithelial phenotype and are particularly useful for studying host-pathogen interactions in the gastrointestinal tract of poultry. LMH cells are adherent and exhibit a dendritic-like morphology. They express glucose-6-phosphatase and weak canalicular ATPase activity. With a triploid karyotype and six marker chromosomes, these cells display distinct genetic characteristics. In terms of tumorigenicity, LMH cells have the ability to form tumors in athymic nude mice. This characteristic makes them an important model for studying hepatocellular carcinoma. LMH cells express the estrogen receptor and can be induced to express the liver-specific apolipoprotein II (apoII) gene. This indicates their involvement in estrogen signaling pathways and lipid metabolism. To culture LMH cells, it is necessary to precoat tissue culture vessels with 0.1% gelatin. This ensures proper cell adhesion and growth. |
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Organism | Chicken |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Applications | The cell line is useful for transfection studies. |
Synonyms | Leghorn Male Hepatoma cell line |
Characteristics
Age | 16 months |
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Gender | Male |
Morphology | Epithelial-like, Dendritic like. |
Growth properties | Adherent. It may take a couple of days until cells grow in fully adherent colonies. |
Identifiers / Biosafety / Citation
Citation | LMH (Cytion catalog number 601411) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Estrogen (low level expression). |
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Tumorigenic | LMH cells form tumors in athymic mice. |
Products | glucose-6-phosphatase, canalicular ATPase activity (weak) |
Karyotype | triploid, modal number = 116, six marker chromosomes |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | LMH cells attach better to tissue culture vessels which have been precoated with Collagen. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 to 3 x 10^4 cells/cm^2 |
Fluid renewal | Every 2 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
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