LCLC-103H Cells
General information
Description | LCLC-103H were established from the pleural effusion by Bepler et al. in 1988. |
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Organism | Human |
Tissue | Lung |
Disease | Large cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | LCLC103H, Large Cell Lung Cancer-103H |
Characteristics
Age | 61 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Pleomorph |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | LCLC-103H (Cytion catalog number 300169) |
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Biosafety level | 1 |
Expression / Mutation
Ploidy status | Aneuploid |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 26 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 0.5 to 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Cells will recover from freezing within 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,12
D13S317: 11
D16S539: 13
D5S818: 12
D7S820: 8,11
TH01: 6
TPOX: 8
vWA: 14,16
D3S1358: 16,17
D21S11: 29,31.2
D18S51: 19
D8S1179: 12,14
FGA: 22
D2S1338: 16,19
D19S433: 15
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