L929 Cells
Key facts about L929 cells
Description | L-929 cells are a fibroblast-like cell line derived from the subcutaneous connective tissue of a 100-day-old male C3H/An mouse. Established in the 1940s, this cell line has become pivotal in various biological and medical research fields due to its robustness, ease of culture, and versatility in applications. L-929 cells are characterized by their spindle-shaped, fibroblastic morphology, and adherent growth. They are widely used in cytotoxicity assays and serve as a standard model to assess the biocompatibility of materials and the toxic effects of various substances, which is particularly relevant in the fields of biomaterials and tissue engineering. L-929 cells are also employed in the study of cytokine activity, especially in assays for necrosis factor (TNF) activity, due to their sensitivity to TNF-induced cytotoxicity. This makes them valuable in immunology and inflammation research. L-929 cells are further utilized in virology as a host for viral replication studies. Their susceptibility to various viruses, such as the infectious bursal disease virus (IBDV), facilitates the investigation of viral life cycles, host-virus interactions, and the efficacy of antiviral compounds. Overall, the L-929 cell line is a valuable resource in scientific research and offers a versatile platform for studies in cytotoxicity, immunology, virology, and biomaterials. |
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Organism | Mouse |
Tissue | Connective tissue, normal, subcutaneous, areolar and adipose |
Synonyms | NCTC clone 929, NCTC 929, NCTC-929, NCTC929, L cell, L cells, L-cell, L-cells, L cell line, L, Strain L-929, L 929, L929, L929(NCTC), Clone 929, Earles's cells, Earle's L cells |
Features of the mouse cell line L929
Age | 100 days |
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Gender | Male |
Morphology | Fibroblast-like |
Cell type | Fibroblast |
Growth properties | Adherent |
Specifications
Citation | L-929 (Cytion catalog number 400260) |
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Biosafety level | 1 |
Genotype
Antigen expression | H-2k |
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Tumorigenic | Yes, in immunosuppressed mice |
Viruses | Ectromelia virus (mousepox): negative |
Virus resistance | poliovirus 1, 2, 3, coxsackievirus B5, polyomavirus |
Reverse transcriptase | positive |
L929 cell purity and Identity checks
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 25 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:8 is recommended |
Seeding density | 2 to 3 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | 24 to 48 hours |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
M_18-3: 16
M_4-2: 20.3
M_6-7: 12
M_3-2: 13,14
M_19-2: 12
M_7-1: 25,26,27
M_1-1: 10
M_8-1: 16
M_2-1: 9
M_15-3: 24.3,25.3,26.3
M_6-4: 17,18
M_11-2: 15,16
M_1-2: 17
M_17-2: 15
M_12-1: 16
M_5-5: 14
M_X-1: 26,27
M_13-1: 17
Human D4/D8: -
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