L-428 Cells
General information
Description | The L428 cell line was established in 1978 from the pleural effusion of a patient suffering from Hodgkin's disease. |
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Organism | Human |
Tissue | Pleural effusion |
Disease | Hodgkin lymphoma |
Synonyms | L-428, L 428 |
Characteristics
Age | 37 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | Lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | L428 (Cytion catalog number 300200) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS, 1 mM sodium pyruvate, 1% NEAA |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 1 x 10^5 cells/ml |
Fluid renewal | Every 3 days |
Freezing recovery | Fast |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10,13
D13S317: 14,14
D16S539: 11,12
D5S818: 11,12
D7S820: 11,11
TH01: 7,9.3
TPOX: 8,9
vWA: 15
D3S1358: 14,18
D21S11: 31.2,31.2
D18S51: 14,14
Penta E: 10,17
Penta D: 8,9
D8S1179: 14,14
FGA: 19,25
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HLA alleles |
A*: 03:01:01
B*: 35:03:01
C*: 04:01:01
DRB1*: 12:01:01
DQA1*: 05:05:01
DQB1*: 03:01:01
DPB1*: 04:01:01
E: 01:03:02
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