KG-1 Cells
General information
Description | The KG-1 cell line was established from a bone marrow aspirate. They do not spontaneously differentiate to granulocyte and macrophage like cells, do not express DR and do not respond to colony stimulating factor (CSF). |
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Organism | Human |
Tissue | Bone marrow |
Disease | Acute myelogenous leukemia |
Synonyms | KG1 |
Characteristics
Age | 59 years |
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Gender | Male |
Ethnicity | Caucasian |
Cell type | Myeloblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | KG-1 (Cytion catalog number 300208) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | HLA A30, A31, B35, Cw4 |
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Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 0, ES-D, 1, Me-2, 1, AK-1, 0, GLO-1, 2 |
Viruses | EBNA (EBNA ): negative |
Reverse transcriptase | Negative |
Handling
Culture Medium | IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 45 hours |
Subculturing | Transfer the cell suspension into sterile centrifuge tubes. Collect the cells by spinning down at 300xg for 3 minutes. Discard the supernatant and resuspend the pelleted cells in fresh cell culture medium. Adjust to an optimal cell density between 1 - 3 x 10^5 cells/ml. Split the cells when a maximum cell density of 1 - 2 x 10^6 cells/ml is reached. |
Split ratio | A ratio of 1:2 is recommended |
Fluid renewal | Every 3 days |
Freezing recovery | Allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 7
D13S317: 11,12
D16S539: 11
D5S818: 13
D7S820: 8,10
TH01: 7,8
TPOX: 7,9
vWA: 14,19
D3S1358: 15,16
D21S11: 28,29
D18S51: 10.2,18
Penta E: 7,13
Penta D: 8,9
D8S1179: 13,14
FGA: 22
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