KATO-III Cells
General information
Organism | Human |
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Tissue | Stomach |
Disease | Adenocarcinoma |
Metastatic site | Pleural effusion |
Synonyms | Kato III, Kato-III, KATO III, KATOIII, KatoIII, KATO 3, JTC-28, Japanese Tissue Culture-28 |
Characteristics
Age | 57 years |
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Gender | Male |
Ethnicity | Asian |
Morphology | Spherical |
Growth properties | Adherent/suspension |
Identifiers / Biosafety / Citation
Citation | KATO-III (Cytion catalog number 300381) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 negative, CEA positive |
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Antigen expression | Blood Type B, Rh+ |
Isoenzymes | PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0742 |
Tumorigenic | Yes, in cheek pouches of anti thymocyte serum treated hamsters, not tumorigenic in nude mice |
Karyotype | The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11,HSR) was present in all metaphases examined, but no double minutes (DM) were detected (Sekiguchi 1978). |
Handling
Culture Medium | Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 36 hours |
Subculturing | Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. |
Split ratio | A ratio of 1:2 to 1:8 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 will result in a confluent monolayer within 2 to 3 days. |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 7,11
D13S317: 8,12
D16S539: 10,12
D5S818: 10,11
D7S820: 8,12
TH01: 7,9
TPOX: 11
vWA: 14,16
D3S1358: 15,16
D21S11: 30,31
D18S51: 12
Penta E: 13,18,19
Penta D: 13,14
D8S1179: 13,14
FGA: 23,24
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HLA alleles |
A*: 02:01:01, 02:07:01
B*: 15:01:01, 46:01:01
C*: 01:02:01, 03:03:01
DRB1*: 08:03:02, 15:01:01G
DQA1*: 01:02:01, 01:03:01
DQB1*: 06:01:01, 06:02:01
DPB1*: 02:01:02, 02:02:01
E: 01:03:02
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