K7M2 wt Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305188

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The K7M2 wt cell line is derived from a murine osteosarcoma and is frequently utilized in cancer research, particularly for studies investigating the pathogenesis and therapeutic response of osteosarcoma. This cell line is characterized by its high metastatic potential, making it an invaluable model for studying the mechanisms underlying cancer metastasis and for testing anti-metastatic agents. K7M2 wt cells display a typical epithelial morphology and exhibit robust growth in vitro, which facilitates various experimental applications including gene expression studies, drug screening, and genetic manipulation. Researchers leverage the K7M2 wt cell line to explore the molecular and cellular processes involved in osteosarcoma progression. Studies often focus on signaling pathways, such as the Wnt/β-catenin and PI3K/AKT pathways, which are crucial in tumor growth and metastasis. The genetic profile of K7M2 wt cells includes alterations common in osteosarcoma, providing insights into the genetic drivers of this malignancy. Furthermore, this cell line is instrumental in preclinical testing of new therapeutic approaches, including targeted therapies and immunotherapies, offering a platform for translating research findings into potential clinical applications.
Organism	Mouse
Tissue	Ascites
Disease	Mouse osteosarcoma
Metastatic site	Lung
Synonyms	K7M2-WT, K7M2

Breed/Subspecies	BALB/c
Age	895 days
Gender	Female
Cell type	Osteoblast
Growth properties	Adherent

Citation	K7M2 wt (Cytion catalog number 305188)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_V455

Receptors expressed	Complement(C3), expressed, Fc receptor, IgG, high affinity I(Fcgr1), expressed
Tumorigenic	Yes

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305188-130323SF	Certificate of Analysis	23. May. 2025	305188

K7M2 wt Cells

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Certificate of Analysis (CoA)