Jiyoye Cells
General information
Description | Established from a 7-year-old boy with Burkitt lymphoma. Epstein-Barr virus positive but according to TRBA 468 stable in the latent phase. The subline P3HR-1, deficient in HLA class II, is also available at CLS (order number 302016). |
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Organism | Human |
Tissue | Lymphatic System |
Disease | B-cell Non-Hodgkin-Lymphoma |
Metastatic site | B-Lymphocyte |
Applications | Analysis of B cell surface antigens, testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms, haplotype standard. |
Synonyms | JIYOYE, Jijoye, JIJOYE, P-2003, P3 (Jiyoye), P-3-Jijoye, P3-Jiyoye, P-3J, P3J, Jiyoye(P-2003), Jiyoye (P-2003), JiyoyeP-2003, OB2, GM04678 |
Characteristics
Age | 7 years |
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Gender | Male |
Ethnicity | African |
Cell type | B lymphocyte |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | Jiyoye (Cytion catalog number 300366) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD10+, CD19+ |
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Karyotype | 46, hypodiploid |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 3 x 10^5 cells/ml |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Fast (48 hours) |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
TH01: 7,9
TPOX: 6,8
vWA: 15,19
D3S1358: 16,17
D21S11: 28,36
D18S51: 12
Penta E: 8,12
Penta D: 2.2,12
D8S1179: 14,15
FGA: 23,24
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HLA alleles |
A*: 03:01:01, 03.01.1900 02:01
B*: 02.01.1900 05:01, 02.01.1900 10:01
C*: 04:01:01
DRB1*: 11:02:01, 15:03:01
DQA1*: 01:02:01, 05:05:01
DQB1*: 03:19:01, 06:02:01
DPB1*: 01:01:01, 02:01:02
E: 01:01, 01:03
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