JAR Cells
General information
Description | The JAR line was established by R.A. Pattillo and associates directly from a trophoblastic tumor of the placenta. |
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Organism | Human |
Tissue | Placenta |
Disease | Choriocarcinoma |
Synonyms | Jar, JAr, JaR |
Characteristics
Age | 24 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | JAR (Cytion catalog number 300221) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 2, AK-1, 1, GLO-1, 1, Phenotype Frequency Product: 0.0002 |
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Products | Estrogen, progesterone, hCG, human chorionic somatomammotropin (placental lactogen), hCG production averages 22.5 ng/ml after reculturing |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 7,10
D13S317: 11
D16S539: 9,10
D5S818: 10,11
D7S820: 10,11
TH01: 6,7
TPOX: 8,11
vWA: 16,18
D3S1358: 14
D21S11: 30
D18S51: 13,17
Penta E: 10,12
Penta D: 9,11
D8S1179: 14,16
FGA: 22
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