IMR-32 Cells
General information
Description | The IMR-32 cell culture shows two cell types, a small neuroblast- like cell is predominant, the other one is a large hyaline fibroblast. |
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Organism | Human |
Tissue | Brain |
Disease | Neuroblastoma |
Metastatic site | Abdomen |
Synonyms | IMR 32, IMR32, Institute for Medical Research-32, GM03320, GM3320C, GM03320D, AG03320, AG3320 |
Characteristics
Age | 13 months |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Cell type | Neuroblast |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | IMR-32 (Cytion catalog number 300148) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B |
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Virus susceptibility | Vesicular stomatitis (Indiana), herpes simplex, vaccinia, coxsackievirus B3, poliovirus 3 (poorly) |
Virus resistance | Echovirus 11 |
Reverse transcriptase | Negative |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11,12
D13S317: 9
D16S539: 8
D5S818: 11,12
D7S820: 9,10
TH01: 7,9.3
TPOX: 11
vWA: 15
D3S1358: 16
D21S11: 30,31
D18S51: 12,15
Penta E: 7,15
Penta D: 11,12
D8S1179: 13
FGA: 21,24
D1S1656: 17,17.3
D6S1043: 14,18
D2S1338: 23,24
D12S391: 19.3,23
D19S433: 14,15
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HLA alleles |
A*: 02:01:01, 24:02:01
B*: 07:02:01, 15:01:01
C*: 03:03:01, 07:02:01
DRB1*: 07:01:01, 13:01:01
DQA1*: 01:03:01, 02:01:01
DQB1*: 03:03:02, 06:03:01
DPB1*: 02:01:02, 04:01:01
E: 01:01, 01:03
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