HuTu-80 Cells
General information
Description | The cells express receptors for bombesin at up to 6000 sites per cell. |
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Organism | Human |
Tissue | Duodenum |
Disease | Adenocarcinoma |
Synonyms | HUTU 80, Hutu 80, HuTu 80, HUTU-80, Hutu-80, HUTU80, HuTu80, Hutu80 |
Characteristics
Age | 53 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HuTu-80 (Cytion catalog number 300218) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | bombesin |
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Antigen expression | Blood Type B, Rh+ |
Isoenzymes | PGM3, 1-2, PGM1, 1-2, ES-D, 1, Me-2, 2, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0017 |
Tumorigenic | Yes, in nude mice. Forms well differentiated papillary adenocarcinoma, (grade I) |
Ploidy status | Aneuploid |
Karyotype | (P12) hypodiploid to hyperdiploid with modal number = 46 |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 26 to 30 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:5 is recommended |
Seeding density | 1 to 2 x 10^4 cells/cm^2 is recommended |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Fast |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11,13
D13S317: 8,11
D16S539: 10,11
D5S818: 12,13
D7S820: 9,11
TH01: 7
TPOX: 9,11
vWA: 16,18
D3S1358: 15,17
D21S11: 31,32.2
D18S51: 14,17
Penta E: 12,18
Penta D: 2.2
D8S1179: 15
FGA: 21,23
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