HuT-78 Cells
General information
Description | Derived from the peripheral blood of a patient with S?zary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut-78. |
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Organism | Human |
Tissue | Blood |
Disease | Mycosis fungoides and Sezary syndrome |
Synonyms | Hut 78, HUT 78, HuT 78, HUT-78, HuT78, Hut78, HUT78, NCI-H78 |
Characteristics
Age | 53 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | T lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | HuT-78 (Cytion catalog number 300338) |
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Biosafety level | 1 |
Depositor | T. Lindl |
Expression / Mutation
Receptors expressed | interleukin-2 (interleukin 2, IL-2) |
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Protein expression | p53 negative |
Antigen expression | CD4 |
Products | interleukin-2 (interleukin 2, IL-2), tumor necrosis factor alpha (TNF alpha) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 1 x 10^5 cells/ml |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Allow the cells to recover from the freezing process for 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11,12
D7S820: 8,11
TH01: 8,9
TPOX: 8,9
vWA: 14,15
D3S1358: 15,16
D21S11: 30
D18S51: 18
Penta E: 13,15
Penta D: 9
D8S1179: 12,14
FGA: 21,25
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HLA alleles |
A*: 01:01:01
B*: 15:01:01
C*: 03:03:02
DRB1*: 04:01:01
DQA1*: 03:01:01
DQB1*: 03:02:01
DPB1*: 04:01:01
E: 01:03:02
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