Hepa 1-6 Cells
General information
Description | This cell line is a derivative of the BW7756 mouse hepatoma. |
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Organism | Mouse |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Synonyms | HEPA 1-6, Hepa-1-6, Hepa1-6 |
Characteristics
Gender | Female |
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Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Hepa 1-6 (Cytion catalog number 400474) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in C57BL/6 mice. |
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Viruses | Ectromelia virus (mousepox): Negative. |
Products | albumin, alpha fetoprotein (AFP, alpha-fetoprotein), albumin, alpha antitrypsin (alpha-1-antitrypsin), amylase |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 25 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A subcultivation ratio of 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Good. Allow cells to recover from the freezing process for 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
M_18-3: 16,17
M_4-2: 18.3,19.3
M_6-7: 15
M_3-2: 10
M_19-2: 10,11
M_7-1: 25,2
M_1-1: 14,15,16
M_8-1: 15
M_2-1: 14
M_15-3: 17,18,19
M_6-4: 18,19
M_11-2: 16
M_1-2: 13
M_17-2: 14
M_12-1: 17
M_5-5: 17
M_X-1: 25
M_13-1: 17.1
Human D4/D8: -
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