Hep-66.3A Cells
General information
Description | Established from the primary hepatocellular carcinoma of B6C3F1 mice. |
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Organism | Mouse |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Synonyms | HEP-66.3A, 66.3A |
Characteristics
Age | Adult |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Hep-66.3A (Cytion catalog number 400206) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | Keratin 8, Keratin 18, Vimentin |
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Tumorigenic | Yes, in B6C3F1 mice |
Mutational profile | p53 wt |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Fluid renewal | Every 3 to 5 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
M_18-3: 16,18
M_4-2: 20.3,21.3
M_6-7: 12,17
M_3-2: 14
M_19-2: 12,13
M_7-1: 26,26.2
M_1-1: 10,16
M_8-1: 16
M_2-1: 9,15
M_15-3: 22.3,25.3
M_6-4: 18
M_11-2: 16
M_1-2: 16,20
M_17-2: 15
M_12-1: 16,17
M_5-5: 15,16
M_X-1: 28
M_13-1: 17
Human D4/D8: -
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