Hep-55.1C Cells
General information
Description | Hep-55.1C cells are derived from C57BL/6J mice and offer a valuable resource for studying hepatocellular carcinoma. The cell line can be used to investigate tumor initiation, progression, metastasis, and response to treatments. By studying these cells, researchers can gain insights into the complex biology of hepatocellular carcinoma and contribute to the development of diagnostic techniques and targeted therapies. |
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Organism | Mouse |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Synonyms | HEP-55.1C, 55.1C |
Characteristics
Age | Adult |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Hep-55.1C (Cytion catalog number 400201) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | Keratin 8, Keratin 18, Vimentin. |
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Tumorigenic | Yes, in C57BL/6J mice |
Mutational profile | p53 wt |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | Start culture from cryovial at a cell density of 3 to 4 x 10^4 cells/cm^2. The cells will recover within 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
M_18-3: 16
M_4-2: 20.3
M_6-7: 17
M_3-2: 14
M_19-2: 13
M_7-1: 26.2,27.2
M_1-1: 16
M_8-1: 16
M_2-1: 15
M_15-3: 22.3
M_6-4: 18
M_11-2: 16
M_1-2: 20
M_17-2: 15
M_12-1: 17
M_5-5: 16,17
M_X-1: 28
M_13-1: 17
Human D4/D8: -
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