HS-683 Cells
General information
Description | Hs 683 cells were isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian. Microvilli but no desmosomes were observed. |
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Organism | Human |
Tissue | Brain |
Disease | Oligodendroglioma |
Synonyms | HS 683, Hs 683, Hs-683, Hs683, HS683, Hs 683.T, HS 683T, Hs683T |
Characteristics
Age | 76 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HS-683 (Cytion catalog number 300213) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B, PGM1, 1, PGM3, 1-2, ES-D, 1, Me-2, 2, AK-1, 1, GLO-1, 2, Phenotype Frequency Product: 0.0029 |
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Tumorigenic | No |
Ploidy status | Aneuploid |
Karyotype | (P15) hypotetraploid with mode = 88, range = 44 to 97, Y chromosomes present |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 45 to 50 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 is recommended |
Seeding density | When seeded at 1 x 10^4 cells/cm^2 the cells will reach 80% confluence within 3 to 4 d. |
Fluid renewal | Every 3 days |
Freezing recovery | After thawing, plate the cells at 4 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 9,13
D13S317: 8,12
D16S539: 9,10
D5S818: 11,12
D7S820: 11
TH01: 6,8
TPOX: 8,11
vWA: 18,20
D3S1358: 14,16
D21S11: 27,33.2
D18S51: 12,14
Penta E: 13,15
Penta D: 13,14
D8S1179: 12,13
FGA: 21.2,22
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HLA alleles |
A*: 32:01:01
B*: 07:02:01, 44:02:01
C*: 05:01:01, 07:02:01
DRB1*: 08:01:01, 12:01:01
DQA1*: 04:01:01, 05:05:01
DQB1*: 03:01:01, 04:02:01
DPB1*: 02:01:02, 03:01:01
E: 01:01:01
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