HROG36 Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | This cell line was isolated from patient tumor tissue by PD Dr. Michael Linnebacher and is one cell line of a series of tumor cell lines which have been established by PD Dr. Michael Linnebacher from Primary CRC resection specimens since 2006. |
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Organism | Human |
Tissue | Brain, R, parietal |
Disease | Glioblastoma (grade III) |
Characteristics
Age | 80 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like, Fibroblast-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HROG36 (Cytion catalog number 300939) |
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Biosafety level | 1 |
Depositor | M. Linnebacher |
Expression / Mutation
Antigen expression | GFAP+ , nestin +, vimentin +, S-100+, GBM+, BTSC+ |
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Mutational profile | IDH 1 & 2 wt, TP53wt, K-Ras wt, B-RAFwt, MGMT CN=0, PTEN I5S |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 35 to 40 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11
D13S317: 11
D16S539: 10,11
D5S818: 11,12
D7S820: 9,12
TH01: 7,10
TPOX: 8,11
vWA: 14
D3S1358: 14,18
D21S11: 29,30
D18S51: 16
Penta E: 5,10
Penta D: 12
D8S1179: 14,15
FGA: 23
D1S1656: 12,17.3
D6S1043: 12,14
D2S1338: 17,23
D12S391: 20,23
D19S433: 15
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HLA alleles |
A*: 02:01:01, 01:01:01
B*: 01.01.1900 16:01, 02.01.1900 07:01
C*: 03:03:01, 03:04:01
DRB1*: 04:04:01, 14:54:01
DQA1*: 01:04:01, 03:01:01
DQB1*: 03:02:01, 05:03:01
DPB1*: 04:01:01G, 06:01:01G
E: 01:01, 01:03
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