HROG04 Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | This is one cell line of a series of tumor cell lines which have been established by PD Dr. Michael Linnebacher from Primary CRC resection specimens since 2006. |
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Organism | Human |
Tissue | Brain, R, frontal |
Disease | Glioblastoma (grade IV) |
Characteristics
Age | 53 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | A mixture of fibroblast-likes and epithelial-like cells |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HROG04 (Cytion catalog number 300932) |
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Biosafety level | 1 |
Depositor | M. Linnebacher |
Expression / Mutation
Antigen expression | HLA-A02 +, MHC class I -MHC class II - , Beta-microglobulin +, HLA-E +, HLA-G -, , MIC A -, MIC-B -, ICAM-1 +, GFAP + , nestin +, vimentin +, S-100 +, GBM +, BTSC + |
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Mutational profile | PTENW274L, 9p212.3(CDKN2A) deleted |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 12
D16S539: 12
D5S818: 12,13
D7S820: 7,10
TH01: 9.3
TPOX: 8,9
vWA: 18
D3S1358: 15
D21S11: 30
D18S51: 15,20
Penta E: 12,18
Penta D: 13
D8S1179: 10,13
FGA: 22
D1S1656: 15.3,16
D6S1043: 11,12
D2S1338: 21,23
D12S391: 18.3,19
D19S433: 14,15.2
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HLA alleles |
A*: 01:01:01, 05:35
B*: 08:01, 03:01:01
C*: 07:01:01, 15:02:01
DRB1*: 03:01:01, 11:01:01
DQA1*: 05:01:01, 05:05:01
DQB1*: 02:01:01, 03:01:01
DPB1*: 01:01:01, 04:01:01
E: 01:01:01
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