HROC348Met Cells
USD$800.00*
Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 106 cells for adherent lines or 5 × 106 cells for suspension lines (refer to the batch CoA for details).
General information
| Description | HROC348Met is a human colorectal carcinoma cell line established from a metachronous liver metastasis of a colorectal adenocarcinoma resected from an adult patient within the HROC (Hansestadt Rostock Colorectal Cancer) model collection. The HROC platform was generated through a standardized biobanking and tumor-modeling pipeline integrating clinical annotation, molecular characterization, patient-derived xenografts (PDX), and corresponding in vitro cultures. HROC348Met represents one of the metastatic models derived from surgically resected colorectal cancer tissue and was established under low-passage conditions to preserve tumor-specific biological features. Within the HROC collection, metastatic specimens - particularly liver metastases - demonstrated a high engraftment efficiency in immunodeficient mice, with an overall PDX take rate of approximately 68% across the cohort, and even higher success for metastatic compared to primary tumors. Multivariate analyses identified nodal involvement and activating mutations in KRAS and BRAF as independent predictors of successful model establishment. The collection encompasses all major molecular subtypes of colorectal carcinoma, including chromosomal instability (CIN), CpG island methylator phenotype (CIMP), microsatellite stable (MSS), and microsatellite instability-high (MSI-H) tumors, ensuring molecular representativeness of advanced-stage disease. HROC348Met was established within this rigorously characterized framework, with clinicopathological and molecular annotation according to standardized protocols. As a metastasis-derived, low-passage colorectal carcinoma model, HROC348Met is suitable for investigations of metastatic tumor biology, genotype-phenotype correlations, and therapeutic response testing in both 2D culture and in vivo PDX settings. The integrated biobank approach underlying its generation ensures availability of matched clinical data and, where applicable, corresponding xenograft material, enabling translational studies in precision oncology and drug-response prediction. |
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| Organism | Human |
| Tissue | Liver metastasis |
| Disease | Adenocarcinoma |
| Metastatic site | Liver |
Characteristics
| Age | 77 years |
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| Gender | Male |
| Ethnicity | Caucasian |
| Growth properties | Adherent |
Regulatory Data
| Citation | HROC348Met (Cytion catalog number 300871) |
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| Biosafety level | 1 |
| NCBI_TaxID | 9606 |
| CellosaurusAccession | CVCL_1U99 |
Biomolecular Data
| MSI-status | MSS |
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Handling
| Culture Medium | DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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| Supplements | Supplement the medium with 10% FBS |
| Dissociation Reagent | Accutase |
| Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
| Fluid renewal | Every 3 to 5 days |
| Freeze medium | As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
| Thawing and Culturing Cells |
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| Incubation Atmosphere | 37°C, 5% CO2, humidified atmosphere. |
| Shipping Conditions | Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. |
| Storage Conditions | For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. |
Quality Control & Molecular Analysis
| Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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