HROC32 Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | This is one cell line of a series of tumor cell lines which have been established by PD Dr. Michael Linnebacher from Primary CRC resection specimens since 2006. |
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Organism | Human |
Tissue | Colon ascendens, UICC IV |
Disease | Primary adenocarcinoma, TNM stage T4N2M1R0L0V1 grading G2, Lk(n) + 9, ? Lk(n) 14 |
Synonyms | HROC32P |
Characteristics
Age | 82 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HROC32 (Cytion catalog number 300818) |
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Biosafety level | 1 |
Depositor | M. Linnebacher |
Expression / Mutation
Protein expression | PTEN |
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Antigen expression | CD15 +, CD24 +, CD44 +, CD55 +, CD58 +, CD50 +, CD 54 +, CD66acde +, CD71 +, CD102 +, CD326 +, CD80 -, CD86 -, EpCAM +, HLA-A2 +, MHC I +, MHC II + (IFN-g pretreated), Her2/neu + |
Tumorigenic | Yes, in immune-suppressed nude mice |
Viruses | Free of human pathogenic viruses SV40, JC/BK, HBV, HCV, HIV. |
Mutational profile | APCwt, p53R282W, K-RasG12A, N-Raswt, H-Raswt SNP rs12628 at codon 27, PIK3CAst, BRafwt |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 30 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:5 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | 1 to 2 weeks |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 14
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 8,11
TH01: 8,9
TPOX: 8,11
vWA: 19
D3S1358: 15,17
D21S11: 31
D18S51: 12
Penta E: 10
Penta D: 12
D8S1179: 12
FGA: 18,19
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HLA alleles |
A*: 01:01, 02:01
B*: 08:01, 03:01
C*: 07:01, 14:02
DRB1*: 03:01, 11:01
DQA1*: 01:02, 05:01
DQB1*: 02:01, 03:01
DPB1*: 01:01, 02:01
E: 01:01, 01:03
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