HROC113 Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | This is one cell line of a series of tumor cell lines which have been established by PD Dr. Michael Linnebacher from Primary CRC resection specimens since 2006. |
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Organism | Human |
Tissue | Colon ascendens, UICC IV |
Disease | Primary adenocarcinoma, TNM stage T4N2M0R0, grading G3, Lk(n) +5, ? Lk(n) 45 |
Synonyms | HROC113P |
Characteristics
Age | 41 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HROC113 (Cytion catalog number 300803) |
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Biosafety level | 1 |
Depositor | M. Linnebacher |
Expression / Mutation
Protein expression | PTEN |
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Tumorigenic | Yes, in immune-suppressed nude mice |
Viruses | Free of human pathogenic viruses SV40, JC/BK, HBV, HCV, HIV. |
Ploidy status | euploid |
Mutational profile | p53 mut, APC mut, K-RasG12VA, N-Raswt, H-Raswt, PIK3CAwt, B-Rafwt |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 23 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | Few days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,12
D5S818: 12,14
D7S820: 12
TH01: 6,7
TPOX: 8,12
vWA: 17
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