HFF-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305790

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	HFF-1 is a human foreskin fibroblast cell line frequently used as a feeder layer for the culture of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). Derived from neonatal dermal tissue, HFF-1 cells provide essential extracellular matrix components and secrete key signaling molecules that promote hESC attachment and partially support their pluripotent state. These fibroblasts have been evaluated for their expression of several pluripotency-supporting growth factors, including TGFβ1, activin A, and fibroblast growth factor 2 (FGF-2), although their effectiveness as feeder cells can vary depending on the specific line and culture conditions. In comparative studies, human foreskin fibroblasts like HFF-1 secrete detectable levels of FGF-2 and activin A, though their secretion levels are generally lower than those observed in mouse embryonic fibroblasts. HFF-1 cells also express BMP-4 mRNA and protein, although secreted levels of BMP-4 dimers are extremely low and often undetectable in conditioned media, likely due to intracellular sequestration or inhibition by gremlin. Importantly, the secretion of growth factors by HFF-1 is modulated by mitotic inactivation (e.g., mitomycin C treatment) and media composition (e.g., KnockOut Serum Replacement vs. fetal bovine serum). The ability of HFF-1 cells to support undifferentiated hESC growth correlates with their secretion of activin A and TGFβ1, although supplementation with exogenous activin A can improve the maintenance of pluripotency markers such as SSEA3 when these cells are used as feeders. Overall, HFF-1 serves as a useful human-derived feeder cell model for stem cell culture systems aiming to reduce xeno-components. However, their capacity to maintain long-term undifferentiated hESC cultures is generally considered less robust than that of mouse-derived feeder cells unless combined with specific growth factor supplementation. Their human origin, however, makes them particularly attractive for clinical and translational stem cell applications where xeno-free conditions are essential.
Organism	Human
Tissue	Foreskin, skin
Synonyms	HFF1

Age	<1 month
Gender	Male
Morphology	Fibroblast
Cell type	Fibroblast of foreskin
Growth properties	Adherent

Citation	HFF-1 (Cytion catalog number 305790)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_3285

Mutational profile

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 15% FBS
Dissociation Reagent	Accutase
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305790-221025	Certificate of Analysis	05. Dec. 2025	305790

HFF-1 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)