HCT-15 Cells
Essential facts about the HCT-15 cell line
Description | The HCT-15 line has the same origin as DLD-1, HRT-18 and HCT-8 cell lines, as shown by Vermeulen et al. 1998. |
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Organism | Human |
Tissue | Colorectal |
Disease | Adenocarcinoma |
Synonyms | HCT 15, HCT.15, HCT15 |
Aspects
Age | 67 years |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent |
Documentation
Citation | HCT-15 (Cytion catalog number 300229) |
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Biosafety level | 1 |
Genetic makeup of the colon cancer cell line HCT-15
Antigen expression | The cells are positive for keratin by immunoperoxidase staining. |
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Tumorigenic | In nude mice |
Viruses | Reverse Transcriptase negative |
Culturing guidelines
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 15 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Seeding density | 1 to 2 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Fast |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control of HCT 15 cells
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
TH01: 7,9.3
TPOX: 8,11
vWA: 18,19
D3S1358: 17
D21S11: 29,32.2
D18S51: 11,17
Penta E: 7,14
Penta D: 9,14
D8S1179: 15
FGA: 22
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