HCC827 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305041

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	HCC827 is a human non-small cell lung cancer cell line derived from the lung adenocarcinoma of a middle-aged female patient. These cells exhibit an epithelial morphology and are often used in research related to the epidermal growth factor receptor (EGFR). HCC827 cells are particularly noted for their sensitivity to tyrosine kinase inhibitors (TKIs), specifically those targeting EGFR mutations. This characteristic makes them a valuable model for studying the molecular mechanisms of lung cancer responsiveness to EGFR inhibitors, as well as for testing the efficacy of new therapeutic agents targeting EGFR-dependent pathways. The cell line is also used to explore the mechanisms of acquired resistance to targeted therapies, which is a significant challenge in the treatment of lung cancer. Studies utilizing HCC827 cells have contributed to a better understanding of the genetic and epigenetic alterations that confer resistance to EGFR inhibitors. These findings have implications for the development of strategies to overcome resistance and improve treatment outcomes in lung cancer patients. Furthermore, the HCC827 cell line serves as a tool for investigating the broader cellular and molecular landscape of lung adenocarcinoma, including studies on cell signaling, tumor microenvironment, and cancer metastasis.
Organism	Human
Tissue	Lung
Disease	Lung adenocarcinoma
Synonyms	HCC-827, HCC 827, HCC0827

Age	39 years
Gender	Female
Morphology	Epithelial
Growth properties	Adherent

Citation	HCC827 (Cytion catalog number 305041)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_2063

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305041-281122	Certificate of Analysis	23. May. 2025	305041
305041-161224	Certificate of Analysis	23. May. 2025	305041

HCC827 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)