HB-CLS-1 Cells
General information
Description | Established as in vitro culture from the primary bladder carcinoma (grade III) of a male patient. |
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Organism | Human |
Tissue | Bladder |
Disease | Carcinoma |
Synonyms | HBCLS1 |
Characteristics
Age | 62 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HB-CLS-1 (Cytion catalog number 300190) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 positive |
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Tumorigenic | Yes, in nude mice |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 12
D13S317: 12,13
D16S539: 8
D5S818: 11
D7S820: 10,11
TH01: 6
TPOX: 8,10
vWA: 15
D3S1358: 16,18
D21S11: 29
D18S51: 14
Penta E: 10
Penta D: 11,12
D8S1179: 12,14
FGA: 19
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HLA alleles |
A*: 31:01:02, 32:01:01
B*: 35:08:01, 51:01:01
C*: 04:01:01, 15:02:01
DRB1*: 01:01:01, 11:01:01
DQA1*: 01:01:01, 05:05:01
DQB1*: 03:01:01, 05:01:01
DPB1*: 04:01:01
E: 01:03:02
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