H4 Cells
General information
Description | Derived from a 37-year-old White male with neuroglioma, these remarkable epithelial cells offer a valuable resource for neuroscience research. H4 cells closely resemble glial cells in vitro and have gained significant recognition for their applicability in studying neuronal impairment and apoptosis. As an ideal model system, H4 cells boast an adherent phenotype, ensuring their easy handling and compatibility with various experimental setups. These cells hold immense potential for multiple applications, including 3D cell culture and neuroscience research. With their origins in the brain tissue of a human host, H4 cells represent a realistic and biologically relevant tool for investigating neural processes. Researchers can harness the power of these cells to unravel intricate mechanisms underlying neuronal function, disorders, and potential therapeutic interventions. One notable advantage of H4 cells lies in their amenability to transient transfection, enabling the introduction of exogenous genetic material into the cells for targeted investigations. This feature facilitates the manipulation of H4 cells, empowering researchers to delve into specific genetic pathways and unravel the mysteries of neurobiology. Our H4 cells product category encompasses an innovative approach to human cell lines, offering a versatile and efficient toolset for advancing scientific discoveries. By leveraging the potential of H4 cells, researchers can propel the field of neuroscience forward, unravelling the complex interplay of neural components and shedding light on the mechanisms of neuroglioma and associated diseases. With their epithelial morphology and glial-like properties, H4 cells represent a crucial stepping stone in understanding the human brain's intricate workings. Engage in groundbreaking research and uncover transformative insights by harnessing the power of H4 cells-the ultimate ally in your pursuit of knowledge in neuroscience and beyond. |
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Organism | Human |
Tissue | Brain |
Disease | Neuroglioma |
Synonyms | H-4 |
Characteristics
Age | 37 years |
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Gender | Male |
Ethnicity | Caucasian |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | H4 (Cytion catalog number 300184) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | pGP9.5 postive, NeuN positive, NSE negative |
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Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1, ES-D, 1, Me-2, 0, AK-1, 1, GLO-1, 2. |
Tumorigenic | No |
Karyotype | modal number = 75. range 45 = 80. Y chromosome present |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10,12
D13S317: 12
D16S539: 11,12
D5S818: 10,12
D7S820: 8,11
TH01: 7,9
TPOX: 8,11
vWA: 14,18
D3S1358: 17,18
FGA: 30,31
D1S1656: 14,16
D6S1043: 5,12
D2S1338: 10,12
D12S391: 14
D19S433: 19,25
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HLA alleles |
A*: 03:01:01, 30:02:01
B*: 08:01:01, 18:01:01
C*: 05:01:01, 07:01:01
DRB1*: 03:01:01
DQA1*: 05:01:01
DQB1*: 02:01:01
DPB1*: 01:01:01, 04:01:01
E: 01:03:02
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