GIMEN Cells
General information
Description | GIMEN cells represent a distinct line of genetically engineered mouse embryonic neurons, meticulously developed to support advanced research in neurobiology and genetic disorders. These cells are derived from mouse embryonic stem cells and are modified to exhibit specific neuronal properties, making them highly suitable for studies on neural development, neurodegenerative diseases, and neuronal function. The GIMEN cell line is characterized by its stable expression of both wild-type and mutant forms of human genes implicated in neurological conditions, allowing researchers to dissect the molecular pathways involved in disease states. These cells are cultured under strictly controlled conditions to maintain their undifferentiated state and neuronal potential. Upon differentiation, GIMEN cells are capable of expressing a variety of neuronal markers, including NeuN, MAP2, and beta-III tubulin, indicating their utility in modeling complex neural behaviors. Furthermore, the GIMEN cells are amenable to a range of experimental manipulations, including gene editing, drug screening, and electrophysiological assessment, facilitating a comprehensive approach to neurological research. Their consistent performance and high genetic fidelity make them a valuable resource for both fundamental neuroscience research and the exploration of therapeutic interventions. |
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Organism | Human |
Tissue | Brain |
Disease | Neuroblastoma |
Metastatic site | Bone marrow |
Synonyms | Gi-ME-N, Gi-MEN, GI-ME-N, Gimen, Gimen1, Gaslini Institute-ME-Neuroblastoma |
Characteristics
Age | 3,5 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | GIMEN (Cytion catalog number 300179) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 25 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Seeding density | 2 to 3 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 12
D16S539: 9,12
D5S818: 12
D7S820: 10,11
TH01: 6,7
TPOX: 11
vWA: 16,19
D3S1358: 14
FGA: 31
D1S1656: 12,17
D6S1043: 15,2
D2S1338: 9,13
D12S391: 10,14
D19S433: 19,22
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HLA alleles |
A*: 02:01:01, 30:01:01
B*: 13:02:01, 18:01:01
C*: 06:02:01, 07:01:09
DRB1*: 04:03:01, 07:01:01
DQA1*: 02:01:01, 03:01:01
DQB1*: 02:02:01, 03:02:01
DPB1*: 02:01:02
E: 01:01:01, 01:xx
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