GH3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300383

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The GH3 cell line, originating from a rat pituitary tumor, is a critical resource in the study of pituitary functions, particularly regarding the secretion of prolactin and growth hormone. These cells possess characteristics of both somatotropic and lactotropic cells, enabling detailed investigations into pituitary hormones and their regulatory mechanisms. The cell line is extensively utilized to understand the effects of hormonal treatments and genetic modifications on the secretion of these hormones. GH3 cells respond significantly to thyroid-stimulating hormones, making them a valuable model for assays that measure the impact of various compounds on pituitary gland activities. Research employing GH3 cells often delves into how these cells react to different hormonal stimuli. For example, hydrocortisone is known to promote growth hormone production while inhibiting prolactin output in these cells, making GH3 a preferred model for exploring hormonal balance and the endocrine system’s response to stress and other physiological factors. Such studies are pivotal in advancing our understanding of pituitary gland disorders and crafting therapies for conditions like growth deficiencies and hyperprolactinemia. Moreover, GH3 cells are instrumental in pharmacological testing and biotechnological applications aimed at developing treatments for pituitary-related disorders. Their ability to produce more growth hormone compared to GH1 cells, along with prolactin, allows researchers to examine the regulation and effects of these hormones under various conditions. This unique profile is essential for understanding the complex interactions within the endocrine system and for the development of targeted therapeutic interventions.
Organism	Rat
Tissue	Brain, Pituitary gland
Disease	Neoplasm
Synonyms	GH 3

Breed/Subspecies	Wistar Furth
Age	7 months
Gender	Female
Morphology	Epithelial-like
Growth properties	Adherent, clusters in suspension

Citation	GH3 (Cytion catalog number 300383)
Biosafety level	1
NCBI_TaxID	10116
CellosaurusAccession	CVCL_0273

Products	Growth hormone, prolactin

Culture Medium	Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3 (Cytion article number 820608a)
Supplements	Supplement the medium with 15% horse serum, 2.5% heat-inactivated FBS
Dissociation Reagent	Accutase
Subculturing	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300383-200223	Certificate of Analysis	26. May. 2025	300383
300383-200223SF	Certificate of Analysis	23. May. 2025	300383
300383-180225	Certificate of Analysis	21. Jul. 2025	300383

GH3 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)