FRTL-5 Cells
General information
Description | FRTL-5 cells, derived from the thyroid glands of Fischer rats aged 5 to 6 weeks, offer a remarkable resource for researchers in biological science. This clonal line of continuously cultured FRTL cells has gained recognition for maintaining highly differentiated thyroid features, including thyroglobulin secretion and iodide concentration. Unlike other cell lines, FRTL-5 cells tend to grow in intricate three-dimensional structures rather than forming a monolayer, providing a unique model for studying thyroid cell behaviour. The breakthroughs achieved using FRTL-5 cells are worth highlighting. Three decades ago, the esteemed Kohn group pioneered a bioassay based on these cells, exhibiting exceptional reproducibility, feasibility, and diagnostic precision. Through this innovative approach, Kohn and colleagues achieved a significant milestone by developing monoclonal antibodies (moAbs) explicitly targeting the thyroid-stimulating hormone receptor (TSHR). This groundbreaking accomplishment shed light on the multifaceted functional nature of thyroid receptor antibodies (TRAbs) in individuals with Graves' disease. Notably, their research unveiled not only stimulating and blocking TRAbs but also antibodies that activated alternative pathways beyond the traditional cyclic adenosine monophosphate (cAMP) pathway. The applications of FRTL-5 cells extend far beyond their pivotal role in deciphering the complexities of Graves' disease. Researchers have relied on these cells to explore the inner workings of thyroid cells, studying hormone dependency and secretion mechanisms. Their unique ability to maintain the characteristics of differentiated thyroid cells provides an invaluable platform for investigating the intricate processes that govern thyroid function. |
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Organism | Rat |
Tissue | Thyroidea |
Synonyms | FRTL 5, FRTL5, FRTL-5 Cl 2 |
Characteristics
Age | 6 weeks |
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Gender | Unspecified |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | FRTL-5 (Cytion catalog number 500407) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) |
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Medium supplements | Supplement the medium with 10% FBS, 10 mg/L Insulin, 5 mg/L Transferrin, 50 microgram/L Hydrocortison, 10 microgram/L Somatostatin, 10 microgram/L Gly-His-Lsy-acetate, 0.0165 microgram/ml TSH (from Scrippslabs) - Add the required TSH just before use and sterile filter into the medium. |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Rat_D1Wox31: 104
Rat_D2Wox37: 150
Rat_D19Wox11: 212
Rat_D10Wox8: 266
Rat_D4Wox7: 153
Rat_D2Wox27: 211
Rat_D5Rat33: 136
Rat_D10Wox11: 165
Rat_D1Wox23: 210
Rat_D12Wox1: 402
Rat_D6Wox2: 112
Rat_D8Wox7: 182
Rat_D6Cebr1: 233
SRY: x,Y
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